Electron beam powder bed fusion (EB-PBF) is a powder-bed fusion additive manufacturing process, which is suitable for fabricating high-performance parts for a wide range of industrial applications, such as medical and aerospace. Due to its deep curing capabilities, the metastable β-alloy Ti-5Al-5Mo-5V-3Cr (Ti-5553) is currently mostly used in the landing gear of airplanes. However, its great mechanical properties make it also attractive for small, complex, and load-bearing components. In this study, nine melting parameter sets, combining different scanning speeds and beam currents, were used in the EB-PBF ARCAM A2X system. Furthermore, the correlation between the microstructure and the mechanical properties was investigated and analyzed by applying µ-focus computer tomography and microscopic methods (optical, SEM/EDS). A significant influence of the different melting parameters on the microstructure as well as on the mechanical performance was found. In a subsequent step, three melting parameters were selected and the specimens were heat-treated (BASCA, STA) for further investigation. The experimental results of this work indicate that Ti-5553 parts can be manufactured successfully with high quality (ρ > 99.60%), and post-processing heat-treatments can be used to modify the microstructure in such a way that the parts are suitable for a large variety of possible applications.