Cardiomyocytes derived from human induced pluripotent stem cells (iPSC-CMs) provide a valuable source of human cardiomyocytes for biomedical studies. To induce iPSC-CM maturation by combining different stimuli, we designed a micro-physiological system (MPS) which mimics heart filling cycles through hemodynamic stimulation. To investigate the effect of geometry and flow conditions on iPSC-CM function we performed experiments comparing four conditions: 6-well static control, geometry static control (identical to cell chambers in the MPS), semistatic-flow MPS chip and continuous-flow MPS chip. Our data demonstrate that semistatic- and continuous-flow groups reveal significantly higher beating duration, velocity, and cell displacement than 6-well and geometry static controls. Importantly, the combination of geometry with cyclic pulsatile flow significantly enhances the beating intensity of iPSC-CMs not only in continuous microfluidic chips but also in semistatic chips, although the pulsatile frequency was lower than in MPS chips. Electrical stimulation of the iPSC-CM in the MPS provides an additional stimulus to induce maturation in future studies.