The influence of non-smooth trachea walls on phonation onset and offset pressures and the fundamental frequency of oscillation were experimentally investigated for three different synthetic vocal fold models. Three models of the trachea were compared: a cylindrical tube (smooth walls) and wavy-walled tubes with ripple depths of 1 and 2 mm. Threshold pressures for the onset and offset of phonation were measured at the lower and upper ends of each trachea tube. All measurements were performed both with and without a supraglottal resonator. While the fundamental frequency was not affected by non-smooth trachea walls, the phonation onset and offset pressures measured right below the glottis decreased with an increasing ripple depth of the trachea walls (up to 20% for 2 mm ripples). This effect was independent from the type of glottis model and the presence of a supraglottal resonator. The pressures at the lower end of the trachea and the average volume velocities showed a tendency to decrease with an increasing ripple depth of the trachea walls but to a much smaller extent. These results indicate that the subglottal geometry and the flow conditions in the trachea can substantially affect the oscillation of synthetic vocal folds.