Dynamic Control of Cell Cycle and Growth Coupling by Ecdysone, EGFR, and PI3K Signaling in Drosophila Histoblasts

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Nikolay Ninov - , Professur für Zellbiologie und Regeneration von Betazellen, Consejo Superior de Investigaciones Científicas, CSIC - Instituto de Biología Molecular de Barcelona (Autor:in)
  • Cristina Manjon - , Consejo Superior de Investigaciones Científicas (Autor:in)
  • Enrique Martin-Blanco - , Consejo Superior de Investigaciones Científicas (Autor:in)

Abstract

Regulation of cell proliferation has been extensively studied in cultured cell systems that are characterized by coordinated growth and cell-cycle progression and relatively uniform cell size distribution. During the development of multicellular organisms, however, growth and division can be temporally uncoupled, and the signaling pathways that regulate these growth programs are poorly understood. A good model for analyzing proliferation control in such systems is the morphogenesis of the Drosophila adult abdominal epidermis by histoblasts. These cells undergo a series of temporally regulated transitions during which neither cell size nor division rate is constant. The proliferation of histoblasts during metamorphosis is uniquely amenable to clonal analysis in combination with live imaging. Thereby, we show that abdominal histoblasts, which grow while in G2 arrest during larval stages, enter a proliferative stage in the pupal period that is initiated by ecdysone-dependent string/Cdc25 phosphatase transcription. The proliferating histoblasts have preaccumulated stores of Cyclin E, which trigger an immediate S phase onset after mitosis. These rapid cell cycles lack a G1 phase and result in a progressive reduction of cell size. Eventually, the histoblasts proceed to a stage of slower proliferation that, in contrast to the preceding, depends on epidermal growth factor receptor (EGFR) signaling for progression through the G2/M transition and on insulin receptor/PI3K-mediated signaling for growth. These results uncover the developmentally programmed changes coupling the growth and proliferation of the histoblasts that form the abdominal epidermis of Drosophila. Histoblasts proceed through three distinct stages: growth without division, division without growth, and growth-coupled proliferation. Our identification of the signaling pathways and cell-cycle regulators that control these programs illustrates the power of in vivo time-lapse analyses after clone induction. It sets the stage for the comprehensive understanding of the coordination of cell growth and cell-cycle progression in complex multicellular eukaryotes.

Details

OriginalspracheEnglisch
Seiten (von - bis)892-903
Seitenumfang12
FachzeitschriftPLoS biology
Jahrgang7
Ausgabenummer4
PublikationsstatusVeröffentlicht - Apr. 2009
Peer-Review-StatusJa

Externe IDs

PubMed 19355788
Scopus 65949088351

Schlagworte

Schlagwörter

  • Phosphoinositide 3-kinase, C-elegans, Receptor, Pathway, Embryogenesis, Proliferation, Survival, Size, Eye, Differentiation

Bibliotheksschlagworte