Dynamic behavior of cell-free mitochondrial DNA in human saliva

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Caroline Trumpff - , Columbia University (Autor:in)
  • Shannon Rausser - , Columbia University (Autor:in)
  • Rachel Haahr - , Columbia University (Autor:in)
  • Kalpita R. Karan - , Columbia University (Autor:in)
  • Gilles Gouspillou - , Université du Québec à Montréal (Autor:in)
  • Eli Puterman - , University of British Columbia (Autor:in)
  • Clemens Kirschbaum - , Professur für Biopsychologie (Autor:in)
  • Martin Picard - , Columbia University (Autor:in)

Abstract

Mitochondria contain their own genome that can be released in multiple biofluids such as blood and cerebrospinal fluid, as cell-free mitochondrial DNA (cf-mtDNA). In clinical studies, blood cf-mtDNA predicts mortality and higher cf-mtDNA levels are associated with mental and physical stress. However, the dynamics of cf-mtDNA has not been defined, and whether it can be measured non-invasively like other neuroendocrine markers in saliva has not been examined. Here we report cf-mtDNA in human saliva and establish its natural within-person dynamic behavior across multiple weeks. In a small proof-of-principle cohort of healthy adults, we first develop an approach to rapidly quantify salivary cf-mtDNA without DNA isolation, and demonstrate the existence of salivary cf-mtDNA. We then deploy this approach to perform an intensive repeated-measures analysis of two healthy men studied at 4 daily timepoints over 53–60 consecutive days (n = 212–220 observations each) with parallel measures of steroid hormones, self-reported daily mood, and health-related behaviors. Salivary cf-mtDNA exhibited a robust awakening response reaching up to two orders of magnitude 30–45 min after awakening, varied from day-to-day, and moderately correlated with the cortisol awakening response. In exploratory analyses, no consistent association with self-reported daily mood/health-related behaviors were found, although this requires further examination in future studies. Dynamic variation in cf-mtDNA was inversely related with salivary interleukin 6 (IL-6), inconsistent with a pro-inflammatory effect of salivary cf-mtDNA. The highly dynamic behavior of salivary cf-mtDNA opens the door to non-invasive studies examining the relevance of mtDNA signaling in relation to human health.

Details

OriginalspracheEnglisch
Aufsatznummer105852
FachzeitschriftPsychoneuroendocrinology
Jahrgang143
PublikationsstatusVeröffentlicht - Sept. 2022
Peer-Review-StatusJa

Externe IDs

PubMed 35834882
WOS 000830860100002

Schlagworte

Forschungsprofillinien der TU Dresden

Fächergruppen, Lehr- und Forschungsbereiche, Fachgebiete nach Destatis

Ziele für nachhaltige Entwicklung

Schlagwörter

  • Awakening response, Cell-free mitochondrial DNA, Saliva, Steroid hormones, Cell-Free Nucleic Acids, Humans, Mitochondria/genetics, Adult, DNA, Mitochondrial, Male, Hydrocortisone/analysis, Saliva/chemistry, Cell -free mitochondrial DNA

Bibliotheksschlagworte