Direct mechanical measurements reveal the material properties of three-dimensional DNA origami

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

The application of three-dimensional DNA origami objects as rigid mechanical mediators or force sensing elements requires detailed knowledge about their complex mechanical properties. Using magnetic tweezers, we directly measure the bending and torsional rigidities of four- and six-helix bundles assembled by this technique. Compared to duplex DNA, we find the bending rigidities to be greatly increased while the torsional rigidities are only moderately augmented. We present a mechanical model explicitly including the crossovers between the individual helices in the origami structure that reproduces the experimentally observed behavior. Our results provide an important basis for the future application of 3D DNA origami in nanomechanics.

Details

OriginalspracheEnglisch
Seiten (von - bis)5558-5563
Seitenumfang6
FachzeitschriftNano letters
Jahrgang11
Ausgabenummer12
PublikationsstatusVeröffentlicht - 14 Dez. 2011
Peer-Review-StatusJa

Externe IDs

Scopus 83655192457
ORCID /0000-0001-5624-1717/work/142239029

Schlagworte

Schlagwörter

  • Biomechanical Phenomena, DNA/chemistry, Microscopy, Atomic Force, Motion, Nanostructures/chemistry, Nucleic Acid Conformation

Bibliotheksschlagworte