Dilution of contact frequency between superenhancers by loop extrusion at interfaces

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Tetsuya Yamamoto - , Nagoya University, Japan Science and Technology Agency (Autor:in)
  • Helmut Schiessel - , Leiden University (Autor:in)

Abstract

The loop extrusion theory predicts that cohesin acts as a molecular motor that extrudes chromatin fibers to produce loops. Hi-C experiments have detected relatively high contact frequencies between superenhancers. These probably result from the fact that superenhancers are localized at condensates of transcriptional activators and coactivators. The contact frequency between superenhancers is enhanced by auxin treatment that removes cohesin from chromatin. Motivated by these experimental results, we here treat chromatin at the surface of a condensate as a loop extruding polymer brush. Our theory predicts that the lateral pressure generated by the brush decreases with decreasing the loading rate of cohesin. This is because loop extrusion actively transfers chain segments at the vicinity of the interface. Our theory thus predicts that the increase of contact frequency by auxin treatment results from the fact that suppressing the loop extrusion process induces the dissolution of molecular components to the nucleoplasm, decreasing the average distance between superenhancers.

Details

OriginalspracheEnglisch
Seiten (von - bis)7635-7643
Seitenumfang9
FachzeitschriftSoft matter
Jahrgang15
Ausgabenummer38
PublikationsstatusVeröffentlicht - 2019
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMed 31482924

Schlagworte