Differentiation of functional endothelial cells from human induced pluripotent stem cells: A novel, highly efficient and cost effective method

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Xiaopeng Liu - , Georg-August-Universität Göttingen (Autor:in)
  • Jing Qi - , Georg-August-Universität Göttingen (Autor:in)
  • Xingbo Xu - , Georg-August-Universität Göttingen, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK) (Autor:in)
  • Michael Zeisberg - , Georg-August-Universität Göttingen, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK) (Autor:in)
  • Kaomei Guan - , Institut für Pharmakologie und Toxikologie, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen (Autor:in)
  • Elisabeth M. Zeisberg - , Georg-August-Universität Göttingen, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK) (Autor:in)

Abstract

Endothelial cells derived from human induced pluripotent stem cells (hiPSC- EC) are of significant value for research on human vascular development, in vitro disease models and drug screening. Here we report an alternative, highly efficient and cost-effective simple three step method (mesoderm induction, endothelial cell differentiation and endothelial cell expansion) to differentiate hiPSC directly into endothelial cells. We demonstrate that efficiency of described method to derive CD31+ and VE-Cadherin+ double positive cells is higher than 80% in 12 days. Most notably we established that hiPSC-EC differentiation efficacy depends on optimization of both mesoderm differentiation and endothelial cell differentiation steps.

Details

OriginalspracheEnglisch
Seiten (von - bis)225-236
Seitenumfang12
FachzeitschriftDifferentiation
Jahrgang92
Ausgabenummer4
PublikationsstatusVeröffentlicht - 1 Okt. 2016
Peer-Review-StatusJa

Externe IDs

Scopus 84971663847
PubMed 27266810

Schlagworte

Ziele für nachhaltige Entwicklung

Schlagwörter

  • EC, Highly efficient differentiation method, hiPSC