Differentiation of functional endothelial cells from human induced pluripotent stem cells: A novel, highly efficient and cost effective method
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Endothelial cells derived from human induced pluripotent stem cells (hiPSC- EC) are of significant value for research on human vascular development, in vitro disease models and drug screening. Here we report an alternative, highly efficient and cost-effective simple three step method (mesoderm induction, endothelial cell differentiation and endothelial cell expansion) to differentiate hiPSC directly into endothelial cells. We demonstrate that efficiency of described method to derive CD31+ and VE-Cadherin+ double positive cells is higher than 80% in 12 days. Most notably we established that hiPSC-EC differentiation efficacy depends on optimization of both mesoderm differentiation and endothelial cell differentiation steps.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 225-236 |
Seitenumfang | 12 |
Fachzeitschrift | Differentiation |
Jahrgang | 92 |
Ausgabenummer | 4 |
Publikationsstatus | Veröffentlicht - 1 Okt. 2016 |
Peer-Review-Status | Ja |
Externe IDs
Scopus | 84971663847 |
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PubMed | 27266810 |
Schlagworte
Ziele für nachhaltige Entwicklung
Schlagwörter
- EC, Highly efficient differentiation method, hiPSC