tBiocatalytic processes can be used for the in situ production of harmful reagents, e.g., percarboxylic acidswhich are intermediates in epoxidations. Chemo-enzymatic epoxidations of unsaturated fatty acids using these enzymatically synthesized peracids are currently receiving increased interest by the academicand industrial community as safe and environmentally friendly alternatives to chemical synthesis. Theapplicability of these reaction cascades is still limited due to enzyme inhibition and deactivation whichimpair the first – enzymatic – step and thus hinder the following chemical step. To overcome theselimitations, a new strategy for the synthesis of peracids was investigated. This is based on a continuousstirred tank reactor and the avoidance of liquid/liquid interfaces by performing the reaction in a single-phase organic solution. With this strategy, full enzymatic activity could be maintained for 50 h, doublingthe literature benchmark. The peroxyacetic acid yield with respect to hydrogen peroxide was up to 70%.The designed process enables high peracid space-time yields with a reduced demand of enzymes.