Cultivation dependent formation of siderophores by Gordonia rubripertincta CWB2

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Ringo Schwabe - , Technische Universität Bergakademie Freiberg (Autor:in)
  • Christoph Helmut Rudi Senges - , Ruhr-Universität Bochum (Autor:in)
  • Julia Elisabeth Bandow - , Ruhr-Universität Bochum (Autor:in)
  • Thomas Heine - , Professur für Molekulare Biotechnologie, Technische Universität Bergakademie Freiberg (Autor:in)
  • Henry Lehmann - , Technische Universität Bergakademie Freiberg (Autor:in)
  • Oliver Wiche - , Technische Universität Bergakademie Freiberg (Autor:in)
  • Michael Schlömann - , Technische Universität Bergakademie Freiberg (Autor:in)
  • Gloria Levicán - , Universidad de Santiago de Chile (Autor:in)
  • Dirk Tischler - , Technische Universität Bergakademie Freiberg, Ruhr-Universität Bochum (Autor:in)

Abstract

Herein we demonstrate cultivation-dependent siderophore production by the actinomycete Gordonia rubripertincta CWB2. The strain produces mostly citrate, but also desferrioxamine E (DFOE) and new hydroxamate-type siderophores. The production of hydroxamate-like siderophores is influenced by cultivation conditions, for example available carbon sources or presence of metals, such as the rare earth erbium or the heavy metal lead. By cultivation with succinate and extraction with an adsorbing resin (XAD) we purified the G. rubripertincta CWB2 siderophores (yield up to 178 mg L−1). The respective workflow comprises genome mining, cultivation, and overproduction strategies, a rapid screening procedure, as well as traditional structure enrichment and structure elucidation methods. This combination of methods allows the discovery of new natural products with metal complexation capacity, also for lanthanides of commercial value. G. rubripertincta CWB2 carries a desferrioxamine-like biosynthetic gene cluster. Its transcription was proven by a transcriptomic approach comparing expression levels of the selected gene cluster during cultivation in iron-depleted and repleted media. Further investigation of the siderophores of this desferrioxamine producing Actinobacterium could lead to new structures.

Details

OriginalspracheEnglisch
Aufsatznummer126481
FachzeitschriftMicrobiological research
Jahrgang238
PublikationsstatusVeröffentlicht - Sept. 2020
Peer-Review-StatusJa

Externe IDs

PubMed 32497965
ORCID /0000-0002-7109-2788/work/142249509

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • Actinobacteria, Chelating agent, Dissolution, Heavy metals, Metallophore, Rare earth elements

Bibliotheksschlagworte