Crosslinked and Multi-Responsive Polymeric Vesicles as a Platform to Study Enzyme-Mediated Undocking Behavior: Toward Future Artificial Organelle Communication

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung



Various cellular functions are successfully mimicked, opening the door to the next generation of therapeutic approaches and systems biology. Herein, the first steps are taken toward the construction of artificial organelles for mimicking cell communication by docking and undocking of cargo in the membrane of swollen artificial organelles. Stimuli-responsive and crosslinked polymeric vesicles are used to allow docking processes at acidic pH at which ferrocene units in the swollen membrane state can undergo desired specific host-guest interaction using beta-cyclodextrin as model cargo. The release of the cargo mediated by two different enzymes, glucose oxidase and alpha-amylase, is investigated, triggered by distinct enzymatic undocking mechanisms. Different release times for a useful transport are shown that can be adapted to different communication pathways. In addition, Forster resonance energy transfer (FRET) experiments further support the hypotheses of host-guest inclusion complexation formation and their time-dependent breakdown. This work paves a way to a platform based on polymeric vesicles for synthetic biology, cell functions mimicking, and the construction of multifunctional cargo delivery system.


FachzeitschriftMacromolecular rapid communications
Frühes Online-DatumMärz 2023
PublikationsstatusVeröffentlicht - Aug. 2023

Externe IDs

PubMed 36755359
Scopus 85150853017
ORCID /0000-0002-4531-691X/work/148608050



  • Artificial organelles, Degradation process, Enzymes, Host-guest interactions, Polymeric vesicles, Redox process, Artificial Cells, Polymers