In this study, the relatively new picture frame method applied to wood is compared with three established shear test methods, namely the experimental modal analysis, the square plate twist method and the torsion test. For the investigations, the wood species European beech (Fagus sylvatica L.) and spruce (Picea abies L. Karst.) were used and the shear tests were conducted in LR and RL direction. The results show comparable shear moduli for beech and spruce in the range of 931–1289 Nmm⁻² and 495–842 Nmm⁻², respectively. In contrast to the theory of linear elastic orthotropic materials, significant differences in the results of the picture frame method between LR and RL direction were observed for spruce.