Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • D F Muris - , Leiden University (Autor:in)
  • O Bezzubova - (Autor:in)
  • J M Buerstedde - (Autor:in)
  • K Vreeken - (Autor:in)
  • A S Balajee - (Autor:in)
  • C J Osgood - (Autor:in)
  • C Troelstra - (Autor:in)
  • J H Hoeijmakers - (Autor:in)
  • K Ostermann - , Umweltmonitoring und Endokrinologie (FoG) (Autor:in)
  • H Schmidt - (Autor:in)

Abstract

The RAD52 gene of Saccharomyces cerevisiae is required for recombinational repair of double-strand breaks. Using degenerate oligonucleotides based on conserved amino acid sequences of RAD52 and rad22, its counterpart from Schizosaccharomyces pombe, RAD52 homologs from man and mouse were cloned by the polymerase chain reaction. DNA sequence analysis revealed an open reading frame of 418 amino acids for the human RAD52 homolog and of 420 amino acid residues for the mouse counterpart. The identity between the two proteins is 69% and the overall similarity 80%. The homology of the mammalian proteins with their counterparts from yeast is primarily concentrated in the N-terminal region. Low amounts of RAD52 RNA were observed in adult mouse tissues. A relatively high level of gene expression was observed in testis and thymus, suggesting that the mammalian RAD52 protein, like its homolog from yeast, plays a role in recombination. The mouse RAD52 gene is located near the tip of chromosome 6 in region G3. The human equivalent maps to region p13.3 of chromosome 12. Until now, this human chromosome has not been implicated in any of the rodent mutants with a defect in the repair of double-strand breaks.

Details

OriginalspracheEnglisch
Seiten (von - bis)295-305
Seitenumfang11
FachzeitschriftMutation research
Jahrgang315
Ausgabenummer3
PublikationsstatusVeröffentlicht - Nov. 1994
Peer-Review-StatusJa

Externe IDs

Scopus 0027997740

Schlagworte

Schlagwörter

  • Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA Repair/genetics, DNA-Binding Proteins/genetics, Fungal Proteins/genetics, Gene Expression, Genes, Fungal/genetics, Humans, Mice, Molecular Sequence Data, Organ Specificity, RNA, Messenger/analysis, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic/genetics, Saccharomyces cerevisiae/genetics, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid

Bibliotheksschlagworte