Characterization of the skin microbiota in bullous pemphigoid patients and controls reveals novel microbial indicators of disease

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Meriem Belheouane - , Max Planck Institute for Evolutionary Biology, Christian-Albrechts-Universität zu Kiel (CAU), Forschungszentrum Borstel- Leibniz Lungenzentrum (Autor:in)
  • Britt M. Hermes - , Max Planck Institute for Evolutionary Biology, Christian-Albrechts-Universität zu Kiel (CAU) (Autor:in)
  • Nina Van Beek - , Universität zu Lübeck (Autor:in)
  • Sandrine Benoit - , Julius-Maximilians-Universität Würzburg (Autor:in)
  • Philippe Bernard - , CHU de Reims (Autor:in)
  • Kossara Drenovska - , Medical University Sofia (Autor:in)
  • Sascha Gerdes - , Christian-Albrechts-Universität zu Kiel (CAU) (Autor:in)
  • Regine Gläser - , Christian-Albrechts-Universität zu Kiel (CAU) (Autor:in)
  • Matthias Goebeler - , Julius-Maximilians-Universität Würzburg (Autor:in)
  • Claudia Günther - , Klinik und Poliklinik für Dermatologie, Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Anabelle von Georg - , Universität zu Lübeck (Autor:in)
  • Christoph M. Hammers - , Universität zu Lübeck (Autor:in)
  • Maike M. Holtsche - , Universität zu Lübeck (Autor:in)
  • Bernhard Homey - , Universitätsklinikum Düsseldorf (Autor:in)
  • Orsolya N. Horváth - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)
  • Franziska Hübner - , Universität zu Lübeck (Autor:in)
  • Beke Linnemann - , Universität zu Lübeck (Autor:in)
  • Pascal Joly - , CHU Hôpitaux de Rouen (Autor:in)
  • Dalma Márton - , Semmelweis University (Autor:in)
  • Aikaterini Patsatsi - , Aristotle University of Thessaloniki (Autor:in)
  • Claudia Pföhler - , Universität des Saarlandes (Autor:in)
  • Miklós Sárdy - , Ludwig-Maximilians-Universität München (LMU), Semmelweis University (Autor:in)
  • Laura Huilaja - , University of Oulu (Autor:in)
  • Snejina Vassileva - , Medical University Sofia (Autor:in)
  • Detlef Zillikens - , Universität zu Lübeck (Autor:in)
  • Saleh Ibrahim - , Universität zu Lübeck, University of Sharjah (Autor:in)
  • Christian D. Sadik - , Universität zu Lübeck (Autor:in)
  • Enno Schmidt - , Universität zu Lübeck (Autor:in)
  • John F. Baines - , Max Planck Institute for Evolutionary Biology, Christian-Albrechts-Universität zu Kiel (CAU) (Autor:in)

Abstract

Introduction: Bullous pemphigoid (BP) is the most common autoimmune blistering disease. It predominately afflicts the elderly and is significantly associated with increased mortality. The observation of age-dependent changes in the skin microbiota as well as its involvement in other inflammatory skin disorders suggests that skin microbiota may play a role in the emergence of BP blistering. We hypothesize that changes in microbial diversity associated with BP might occur before the emergence of disease lesions, and thus could represent an early indicator of blistering risk. Objectives: The present study aims to investigate potential relationships between skin microbiota and BP and elaborate on important changes in microbial diversity associated with blistering in BP. Methods: The study consisted of an extensive sampling effort of the skin microbiota in patients with BP and age- and sex-matched controls to analyze whether intra-individual, body site, and/or geographical variation correlate with changes in skin microbial composition in BP and/or blistering status. Results: We find significant differences in the skin microbiota of patients with BP compared to that of controls, and moreover that disease status rather than skin biogeography (body site) governs skin microbiota composition in patients with BP. Our data reveal a discernible transition between normal skin and the skin surrounding BP lesions, which is characterized by a loss of protective microbiota and an increase in sequences matching Staphylococcus aureus, a known inflammation-promoting species. Notably, Staphylococcus aureus is ubiquitously associated with BP disease status, regardless of the presence of blisters. Conclusion: The present study suggests Staphylococcus aureus may be a key taxon associated with BP disease status. Importantly, we however find contrasting patterns in the relative abundances of Staphylococcus hominis and Staphylococcus aureus reliably discriminate between patients with BP and matched controls. This may serve as valuable information for assessing blistering risk and treatment outcomes in a clinical setting.

Titel in Übersetzung
Die Charakterisierung der Hautmikrobiota bei Patienten und Kontrollpersonen mit bullösem Pemphigoid deckt neue mikrobielle Krankheitsindikatoren auf

Details

OriginalspracheEnglisch
Seiten (von - bis)71-79
Seitenumfang9
FachzeitschriftJournal of Advanced Research
Jahrgang44 (2023)
Frühes Online-Datum4 Apr. 2022
PublikationsstatusVeröffentlicht - Feb. 2023
Peer-Review-StatusJa

Externe IDs

PubMed 35581140
ORCID /0000-0002-4330-1861/work/151982044

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • 16s rRNA gene sequencing, Autoimmune blistering disease, Bullous pemphigoid, Risk factor, Skin microbiota