Characterization of human oxidoreductases involved in aldehyde odorant metabolism

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Valentin Boichot - , L'Institut Agro Dijon (Autor:in)
  • Franck Menetrier - , L'Institut Agro Dijon (Autor:in)
  • Jean Michel Saliou - , Université de Lille (Autor:in)
  • Frederic Lirussi - , INSERM - Institut national de la santé et de la recherche médicale, Université Bourgogne Franche-Comté, Université de Franche-Comté (Autor:in)
  • Francis Canon - , L'Institut Agro Dijon (Autor:in)
  • Mireille Folia - , Université de Bourgogne (Autor:in)
  • Jean Marie Heydel - , L'Institut Agro Dijon (Autor:in)
  • Thomas Hummel - , Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Susanne Menzel - , Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Maria Steinke - , Julius-Maximilians-Universität Würzburg, Fraunhofer-Institut für Silicatforschung (Autor:in)
  • Stephan Hackenberg - , Rheinisch-Westfälische Technische Hochschule Aachen (Autor:in)
  • Mathieu Schwartz - , L'Institut Agro Dijon (Autor:in)
  • Fabrice Neiers - , L'Institut Agro Dijon (Autor:in)

Abstract

Oxidoreductases are major enzymes of xenobiotic metabolism. Consequently, they are essential in the chemoprotection of the human body. Many xenobiotic metabolism enzymes have been shown to be involved in chemosensory tissue protection. Among them, some were additionally shown to be involved in chemosensory perception, acting in signal termination as well as in the generation of metabolites that change the activation pattern of chemosensory receptors. Oxidoreductases, especially aldehyde dehydrogenases and aldo–keto reductases, are the first barrier against aldehyde compounds, which include numerous odorants. Using a mass spectrometry approach, we characterized the most highly expressed members of these families in the human nasal mucus sampled in the olfactory vicinity. Their expression was also demonstrated using immunohistochemistry in human epitheliums sampled in the olfactory vicinity. Recombinant enzymes corresponding to three highly expressed human oxidoreductases (ALDH1A1, ALDH3A1, AKR1B10) were used to demonstrate the high enzymatic activity of these enzymes toward aldehyde odorants. The structure‒function relationship set based on the enzymatic parameters characterization of a series of aldehyde odorant compounds was supported by the X-ray structure resolution of human ALDH3A1 in complex with octanal.

Details

OriginalspracheEnglisch
Aufsatznummer4876
FachzeitschriftScientific reports
Jahrgang13
Ausgabenummer1
PublikationsstatusVeröffentlicht - 25 März 2023
Peer-Review-StatusJa

Externe IDs

PubMed 36966166
ORCID /0000-0001-7650-1731/work/146644564
ORCID /0000-0001-9713-0183/work/146645374

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • Humans, Oxidoreductases/metabolism, Odorants/analysis, Xenobiotics/metabolism, Smell/physiology, Respiratory System/metabolism, Alcohol Oxidoreductases/metabolism, Receptors, Odorant/genetics