Objective: Motivational Interviewing (MI) is an evidence-based approach to enhance patients' motivation and is increasingly in demand in medical practice. Online teaching methods offer advantages to train health care professionals, but only very few studies examined whether MI-specific interviewing skills (“MI-skills”), and especially MIspecific attitudes (the “MI-spirit”), can be taught online and whether learning gains differ from those in face-to-face courses. This study compared the effects of 7 h of basic training for experienced general practitioners (GP) in either an online or a traditional face-to-face format with a non-trained control group. Methods: “MI-skills” and “MI-spirit” were measured in a prospective analysis in 49 experienced GPs before and one week after training Results: An ANOVA on gain-scores demonstrated significant differences between groups in both MI-specific skills (VASE-R, p = 0.006) and “MIspirit” (MIKAT, p = 0.029; HRQ, p < 0.001) from pre- to posttest. Post-hoc comparisons revealed significant improvement only in the training groups (VASE-R, p < 0.001; MIKAT, p = 0.014 for online, p = 0.003 for face-to-face; HRQ, p < 0.001). Conclusion: The results suggest similar effects of both online and face-to-face training of “MI-skills” and “MI-spirit” to GPs. Practice Implication: Online learning opportunities should be integrated in MIT programs for general practitioners where appropriate.