This work examines the release rates in different solvents (polar and non-polar) of seabuckthorn oil encapsulated in different natural biopolymers (alginate, k-carrageenan and chitosan). The sea buckthorn oil was encapsulated using the ionotropically crosslinked gelation technique, and spherical orange beads with diameters ranging from 2 to 3 cm were obtained. The free and encapsulated seabuckthorn oil beads were characterized by Fourier transformed infrared (FTIR) spectroscopy. Determination of release rates of seabuckthorn oil (in hexane and methanol) was monitored by UV-Vis spectroscopy. Our experimental data indicated that release rates are determined by the diffusivity in the biopolymer matrices, and the solvent type, which influences the swelling of the beads.