Bioassociation of U(VI) and Eu(III) by Plant (Brassica napus) Suspension Cell Cultures - A Spectroscopic Investigation
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
In this study, we investigated the interaction of U(VI) and Eu(III) with Brassica napus suspension plant cells as a model system. Concentration-dependent (0-200 μM) bioassociation experiments showed that more than 75% of U(VI) and Eu(III) were immobilized by the cells. In addition to this phenomenon, time-dependent studies for 1 to 72 h of exposure showed a multistage bioassociation process for cells that were exposed to 200 μM U(VI), where, after initial immobilization of U(VI) within 1 h of exposure, it was released back into the culture medium starting within 24 h. A remobilization to this extent has not been previously observed. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to correlate the bioassociation behavior of Eu and U with the cell vitality. Speciation studies by spectroscopy and in silico methods highlighted various U and Eu species over the course of exposure. We were able to observe a new U species, which emerged simultaneously with the remobilization of U back into the solution, which we assume to be a U(VI) phosphate species. Thus, the interaction of U(VI) and Eu(III) with released plant metabolites could be concluded.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 6718-6728 |
Seitenumfang | 11 |
Fachzeitschrift | Environmental Science and Technology |
Jahrgang | 55 |
Ausgabenummer | 10 |
Publikationsstatus | Veröffentlicht - 18 Mai 2021 |
Peer-Review-Status | Ja |
Extern publiziert | Ja |
Externe IDs
PubMed | 33929840 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- bioassociation, Brassica napus, europium, plant cells, radionuclides, speciation, time-resolved laser-induced fluorescence spectroscopy, uranium