Bioassociation of U(VI) and Eu(III) by Plant (Brassica napus) Suspension Cell Cultures - A Spectroscopic Investigation

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung


  • Jenny Jessat - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • Susanne Sachs - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • Henry Moll - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • Warren John - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • Robin Steudtner - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • René Hübner - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • Frank Bok - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)
  • Thorsten Stumpf - , Helmholtz-Zentrum Dresden-Rossendorf (Autor:in)


In this study, we investigated the interaction of U(VI) and Eu(III) with Brassica napus suspension plant cells as a model system. Concentration-dependent (0-200 μM) bioassociation experiments showed that more than 75% of U(VI) and Eu(III) were immobilized by the cells. In addition to this phenomenon, time-dependent studies for 1 to 72 h of exposure showed a multistage bioassociation process for cells that were exposed to 200 μM U(VI), where, after initial immobilization of U(VI) within 1 h of exposure, it was released back into the culture medium starting within 24 h. A remobilization to this extent has not been previously observed. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to correlate the bioassociation behavior of Eu and U with the cell vitality. Speciation studies by spectroscopy and in silico methods highlighted various U and Eu species over the course of exposure. We were able to observe a new U species, which emerged simultaneously with the remobilization of U back into the solution, which we assume to be a U(VI) phosphate species. Thus, the interaction of U(VI) and Eu(III) with released plant metabolites could be concluded.


Seiten (von - bis)6718-6728
FachzeitschriftEnvironmental Science and Technology
PublikationsstatusVeröffentlicht - 18 Mai 2021
Extern publiziertJa

Externe IDs

PubMed 33929840


ASJC Scopus Sachgebiete


  • bioassociation, Brassica napus, europium, plant cells, radionuclides, speciation, time-resolved laser-induced fluorescence spectroscopy, uranium