Assessing Biomarkers of Porcine Kidneys under Normothermic Machine Perfusion - Can We Gain Insight into a Marginal Organ?

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

To identify potentially transplantable organs in a pool of marginal kidneys, 33 porcine slaughterhouse kidneys were perfused for 4 h with whole blood. During the normothermic perfusion, plasma, urine, and tissue samples were taken. Several biomarkers for tubule injury, endothelial activation, and inflammatory response were evaluated for a potential correlation with macroscopic
appearance, histology, and filtration activity. Generally, biomarker levels increased during perfusion. TLR-4, EDN-1, and NGAL were not associated with any classification. In contrast, a steeper increase in NAG and IL-6 in plasma correlated with a poor macroscopic appearance at 4 h, indicating a higher
inflammatory response in the kidneys with worse macroscopy early on, potentially due to more damage at the tubules. Although long-term effects on the graft could not be assessed in this setting, early observation under machine perfusion with whole blood was feasible. It allowed the assessment of kidneys under conditions comparable to reperfusion. This setting could give surgeons further insight into the quality of marginal kidneys and an opportunity to pre-treat them.

Details

OriginalspracheEnglisch
Aufsatznummer10280
Seiten (von - bis)1 - 15
Seitenumfang15
FachzeitschriftInternational Journal of Molecular Sciences
Jahrgang25
Ausgabenummer19
PublikationsstatusVeröffentlicht - 24 Sept. 2024
Peer-Review-StatusJa

Externe IDs

ORCID /0009-0000-8786-5338/work/168716733
ORCID /0000-0002-9011-0822/work/168720453

Schlagworte

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Schlagwörter

  • Normothermic machine perfusion, biomarker, kidney, pig, reperfusion injury, biomarker, kidney, normothermic machine perfusion, pig, reperfusion injury