Assessing Biomarkers of Porcine Kidneys under Normothermic Machine Perfusion - Can We Gain Insight into a Marginal Organ?
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
To identify potentially transplantable organs in a pool of marginal kidneys, 33 porcine slaughterhouse kidneys were perfused for 4 h with whole blood. During the normothermic perfusion, plasma, urine, and tissue samples were taken. Several biomarkers for tubule injury, endothelial activation, and inflammatory response were evaluated for a potential correlation with macroscopic
appearance, histology, and filtration activity. Generally, biomarker levels increased during perfusion. TLR-4, EDN-1, and NGAL were not associated with any classification. In contrast, a steeper increase in NAG and IL-6 in plasma correlated with a poor macroscopic appearance at 4 h, indicating a higher
inflammatory response in the kidneys with worse macroscopy early on, potentially due to more damage at the tubules. Although long-term effects on the graft could not be assessed in this setting, early observation under machine perfusion with whole blood was feasible. It allowed the assessment of kidneys under conditions comparable to reperfusion. This setting could give surgeons further insight into the quality of marginal kidneys and an opportunity to pre-treat them.
appearance, histology, and filtration activity. Generally, biomarker levels increased during perfusion. TLR-4, EDN-1, and NGAL were not associated with any classification. In contrast, a steeper increase in NAG and IL-6 in plasma correlated with a poor macroscopic appearance at 4 h, indicating a higher
inflammatory response in the kidneys with worse macroscopy early on, potentially due to more damage at the tubules. Although long-term effects on the graft could not be assessed in this setting, early observation under machine perfusion with whole blood was feasible. It allowed the assessment of kidneys under conditions comparable to reperfusion. This setting could give surgeons further insight into the quality of marginal kidneys and an opportunity to pre-treat them.
Details
Originalsprache | Englisch |
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Aufsatznummer | 10280 |
Seiten (von - bis) | 1 - 15 |
Seitenumfang | 15 |
Fachzeitschrift | International Journal of Molecular Sciences |
Jahrgang | 25 |
Ausgabenummer | 19 |
Publikationsstatus | Veröffentlicht - 24 Sept. 2024 |
Peer-Review-Status | Ja |
Externe IDs
ORCID | /0009-0000-8786-5338/work/168716733 |
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ORCID | /0000-0002-9011-0822/work/168720453 |
Schlagworte
Forschungsprofillinien der TU Dresden
DFG-Fachsystematik nach Fachkollegium
Fächergruppen, Lehr- und Forschungsbereiche, Fachgebiete nach Destatis
Ziele für nachhaltige Entwicklung
ASJC Scopus Sachgebiete
Schlagwörter
- Normothermic machine perfusion, biomarker, kidney, pig, reperfusion injury, biomarker, kidney, normothermic machine perfusion, pig, reperfusion injury