Ase1 selectively increases the lifetime of antiparallel microtubule overlaps

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Jochen Krattenmacher - , Center for Molecular Bioengineering (B CUBE), Czech Academy of Sciences, Karlsuniversität Prag (Autor:in)
  • Manuel Lera-Ramirez - , Sorbonne Université (Autor:in)
  • Alexandre Beber - , Czech Academy of Sciences (Autor:in)
  • Stepan Herynek - , Czech Academy of Sciences (Autor:in)
  • Lenka Grycova - , Czech Academy of Sciences (Autor:in)
  • Xiaocheng Liu - , Northwestern Polytechnical University Xian (Autor:in)
  • Pavel Neuzil - , Northwestern Polytechnical University Xian (Autor:in)
  • Francois Nedelec - , University of Cambridge (Autor:in)
  • Stefan Diez - , Exzellenzcluster PoL: Physik des Lebens, Professur für BioNano-Werkzeuge, Max Planck Institute of Molecular Cell Biology and Genetics (Autor:in)
  • Marcus Braun - , Czech Academy of Sciences (Autor:in)
  • Zdenek Lansky - , Czech Academy of Sciences (Autor:in)

Abstract

Microtubules (MTs) are dynamically unstable polar biopolymers switching between periods of polymerization and depolymerization, with the switch from the polymerization to the depolymerization phase termed catastrophe and the reverse transition termed rescue.1 In presence of MT-crosslinking proteins, MTs form parallel or anti-parallel overlaps and self-assemble reversibly into complex networks, such as the mitotic spindle. Differential regulation of MT dynamics in parallel and anti-parallel overlaps is critical for the self-assembly of these networks.23 Diffusible MT crosslinkers of the Ase1/MAP65/PRC1 family associate with different affinities to parallel and antiparallel MT overlaps, providing a basis for this differential regulation.4567891011 Ase1/MAP65/PRC1 family proteins directly affect MT dynamics12 and recruit other proteins that locally alter MT dynamics, such as CLASP or kinesin-4.713141516 However, how Ase1 differentially regulates MT stability in parallel and antiparallel bundles is unknown. Here, we show that Ase1 selectively promotes antiparallel MT overlap longevity by slowing down the depolymerization velocity and by increasing the rescue frequency, specifically in antiparallelly crosslinked MTs. At the retracting ends of depolymerizing MTs, concomitant with slower depolymerization, we observe retention and accumulation of Ase1 between crosslinked MTs and on isolated MTs. We hypothesize that the ability of Ase1 to reduce the dissociation of tubulin subunits is sufficient to promote its enrichment at MT ends. A mathematical model built on this idea shows good agreement with the experiments. We propose that differential regulation of MT dynamics by Ase1 contributes to mitotic spindle assembly by specifically stabilizing antiparallel overlaps, compared to parallel overlaps or isolated MTs.

Details

OriginalspracheEnglisch
Seiten (von - bis)4071-4080.e6
FachzeitschriftCurrent biology
Jahrgang34
Ausgabenummer17
PublikationsstatusAngenommen/Im Druck - 2024
Peer-Review-StatusJa

Schlagworte

Schlagwörter

  • Ase1/PRC1/MAP65 crosslinkers, diffusible microtubule crosslinkers, microtubule arrays, microtubule dynamics, microtubule overlap stability, microtubules