Analyzing the catalytic processes of immobilized redox enzymes by vibrational spectroscopies

Publikation: Beitrag in FachzeitschriftÜbersichtsartikel (Review)BeigetragenBegutachtung


  • Murat Sezer - , Technische Universität Berlin (Autor:in)
  • Diego Millo - , Technische Universität Berlin, Vrije Universiteit Amsterdam (VU) (Autor:in)
  • Inez M. Weidinger - , Technische Universität Berlin (Autor:in)
  • Ingo Zebger - , Technische Universität Berlin (Autor:in)
  • Peter Hildebrandt - , Technische Universität Berlin (Autor:in)


Analyzing the structure and function of redox enzymes attached to electrodes is a central challenge in many fields of fundamental and applied life science. Electrochemical techniques such as cyclic voltammetry which are routinely used do not provide insight into the molecular structure and reaction mechanisms of the immobilized proteins. Surface-enhanced infrared absorption (SEIRA) and surface-enhanced resonance Raman (SERR) spectroscopy may fill this gap, if nanostructured Au or Ag are used as conductive support materials. In this account, we will first outline the principles of the methodology including a description of the most important strategies for biocompatible protein immobilization. Subsequently, we will critically review SERR and SEIRA spectroscopic approaches to characterize the protein and active site structure of the immobilized enzymes. Special emphasis is laid on the combination of surface-enhanced vibrational spectroscopies with electrochemical methods to analyze equilibria and dynamics of the interfacial redox processes. Finally, we will assess the potential of SERR and SEIRA spectroscopy for in situ investigations on the basis of the first promising studies on human sulfite oxidase and hydrogenases under turnover conditions.


Seiten (von - bis)455-464
FachzeitschriftIUBMB Life
PublikationsstatusVeröffentlicht - Juni 2012
Extern publiziertJa

Externe IDs

PubMed 22535701



  • cyclic voltammetry, electrocatalysis, enzyme immobilization, electron transfer, surface-enhanced infrared absorption spectroscopy, surface-enhanced resonance Raman spectroscopy