Additive manufacturing of collagen scaffolds by three-dimensional plotting of highly viscous dispersions

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Anja Lode - , Zentrum für Translationale Knochen-, Gelenk- und Weichgewebeforschung, Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Michael Meyer - , FILK Freiberg Institute gGmbH (Autor:in)
  • Sophie Brüggemeier - , Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Birgit Paul - , Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)
  • Hagen Baltzer - , FILK Freiberg Institute gGmbH (Autor:in)
  • Michaela Schröpfer - , FILK Freiberg Institute gGmbH (Autor:in)
  • Claudia Winkelmann - , Fraunhofer-Institut für Werkstoff- und Strahltechnik (Autor:in)
  • Frank Sonntag - , Fraunhofer-Institut für Werkstoff- und Strahltechnik (Autor:in)
  • Michael Gelinsky - , Zentrum für Translationale Knochen-, Gelenk- und Weichgewebeforschung, Universitätsklinikum Carl Gustav Carus Dresden (Autor:in)

Abstract

Additive manufacturing (AM) allows the free form fabrication of three-dimensional (3D) structures with distinct external geometry, fitting into a patient-specific defect, and defined internal pore architecture. However, fabrication of predesigned collagen scaffolds using AM-based technologies is challenging due to the low viscosity of collagen solutions, gels or dispersions commonly used for scaffold preparation. In the present study, we have developed a straightforward method which is based on 3D plotting of a highly viscous, high density collagen dispersion. The swollen state of the collagen fibrils at pH4 enabled the homogenous extrusion of the material, the deposition of uniform strands and finally the construction of 3D scaffolds. Stabilization of the plotted structures was achieved by freeze-drying and chemical crosslinking with the carbodiimide EDC. The scaffolds exhibited high shape and dimensional fidelity and a hierarchical porosity consisting of macropores generated by strand deposition as well as an interconnected microporosity within the strands as result of the freeze-drying process. Cultivation of human mesenchymal stromal cells on the scaffolds, with and without adipogenic or osteogenic stimulation, revealed their cytocompatibility and potential applicability for adipose and bone tissue engineering.

Details

OriginalspracheEnglisch
Aufsatznummer015015
FachzeitschriftBiofabrication
Jahrgang8
Ausgabenummer1
PublikationsstatusVeröffentlicht - 2016
Peer-Review-StatusJa

Externe IDs

PubMed 26924825
ORCID /0000-0001-9075-5121/work/160951471

Schlagworte

Schlagwörter

  • Additive manufacturing, Collagen, Extrusion, Freeze-drying, Rapid prototyping