Active fluctuations of axoneme oscillations scale with number of dynein motors

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Abstract

Fluxes of energy generate active forces in living matter, yet also active fluctuations. As a canonical example, collections of molecular motors exhibit spontaneous oscillations with frequency jitter caused by nonequilibrium phase fluctuations. We investigate phase fluctuations in reactivated Chlamydomonas reinhardtii axonemes, which are accessible to direct manipulation. We quantify the precision of axonemal oscillations after controlled chemical removal of dynein motors, providing an experimental test for the theory prediction that the quality factor of motor oscillations should increase with motor number. Our quantification reveals specialized roles of inner and outer arm dynein motors. This supports a model in which inner dyneins serve as master pace-makers, to which outer arm dyneins become entrained, consistent with recent insight provided by structural biology.

Details

OriginalspracheEnglisch
Aufsatznummere2406244121
Seitenumfang9
FachzeitschriftProceedings of the National Academy of Sciences of the United States of America
Jahrgang121 (2024)
Ausgabenummer46
PublikationsstatusVeröffentlicht - 5 Nov. 2024
Peer-Review-StatusJa

Externe IDs

PubMed 39499635
ORCID /0000-0003-1884-2284/work/173054808

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • axoneme, cilia and flagella, molecular motor, nonequilibrium fluctuation, oscillation