Active fluctuations of axoneme oscillations scale with number of dynein motors
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Fluxes of energy generate active forces in living matter, yet also active fluctuations. As a canonical example, collections of molecular motors exhibit spontaneous oscillations with frequency jitter caused by nonequilibrium phase fluctuations. We investigate phase fluctuations in reactivated Chlamydomonas reinhardtii axonemes, which are accessible to direct manipulation. We quantify the precision of axonemal oscillations after controlled chemical removal of dynein motors, providing an experimental test for the theory prediction that the quality factor of motor oscillations should increase with motor number. Our quantification reveals specialized roles of inner and outer arm dynein motors. This supports a model in which inner dyneins serve as master pace-makers, to which outer arm dyneins become entrained, consistent with recent insight provided by structural biology.
Details
Originalsprache | Englisch |
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Aufsatznummer | e2406244121 |
Seitenumfang | 9 |
Fachzeitschrift | Proceedings of the National Academy of Sciences of the United States of America |
Jahrgang | 121 (2024) |
Ausgabenummer | 46 |
Publikationsstatus | Veröffentlicht - 5 Nov. 2024 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 39499635 |
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ORCID | /0000-0003-1884-2284/work/173054808 |
Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- axoneme, cilia and flagella, molecular motor, nonequilibrium fluctuation, oscillation