Laser-plasma accelerators (LPAs) can deliver pico- to nanosecond long proton bunches with ≳100 nC of charge dispersed over a broad energy spectrum. Increasing the repetition rates of today’s LPAs is a necessity for their practical application. This, however, creates a need for real-time proton bunch diagnostics. Scintillating screens are one detector solution commonly applied in the field of electron LPAs for spatially resolved particle and radiation detection. Yet their establishment for LPA proton detection is only slowly taking off, also due to the lack of available calibrations. In this paper, we present an absolute proton number calibration for the scintillating screen type DRZ High (Mitsubishi Chemical Corporation, Düsseldorf, Germany), one of the most sensitive screens according to calibrations for relativistic electrons and x rays. The presented absolute light yield calibration shows an uncertainty of the proton number of 10% and can seamlessly be applied at other LPA facilities. For proton irradiation of the DRZ High screen, we find an increase in light yield of > 60% compared to reference calibration data for relativistic electrons. Moreover, we investigate the scintillating screen light yield dependence on proton energy since many types of scintillators (e.g., plastic, liquid, and inorganic) show a reduced light yield for increased local energy deposition densities, an effect termed ionization quenching. The ionization quenching can reduce the light yield for low-energy protons by up to ∼20%. This work provides all necessary data for absolute spectral measurements of LPA protons with DRZ High scintillating screens, e.g., when used in the commonly applied Thomson parabola spectrometers.