A novel methyltransferase from the intracellular pathogen Plasmodiophora brassicae methylates salicylic acid

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Jutta Ludwig-Müller - , Institut für Botanik, Technische Universität Dresden (Autor:in)
  • Sabine Jülke - , Institut für Botanik, Technische Universität Dresden (Autor:in)
  • Kathleen Geiß - , Technische Universität Dresden (Autor:in)
  • Franziska Richter - , Technische Universität Dresden (Autor:in)
  • Axel Mithöfer - , Max Planck Institute for Chemical Ecology (Autor:in)
  • Ivana Šola - , Technische Universität Dresden, University of Zagreb (Autor:in)
  • Gordana Rusak - , University of Zagreb (Autor:in)
  • Sandi Keenan - , Plant and Food Research, New Zealand (Autor:in)
  • Simon Bulman - , Plant and Food Research, New Zealand (Autor:in)

Abstract

The obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid (SA) production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae seems to suppress plant defence reactions, but information on how this is achieved is scarce. Here, we profile the changes in SA metabolism during Arabidopsis clubroot disease. The accumulation of SA and the emission of methylated SA (methyl salicylate, MeSA) were observed in P.brassicae-infected Arabidopsis 28 days after inoculation. There is evidence that MeSA is transported from infected roots to the upper plant. Analysis of the mutant Atbsmt1, deficient in the methylation of SA, indicated that the Arabidopsis SA methyltransferase was not responsible for alterations in clubroot symptoms. We found that P.brassicae possesses a methyltransferase (PbBSMT) with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when SA production is highest. By heterologous expression and enzymatic analyses, we showed that PbBSMT can methylate SA, benzoic and anthranilic acids.

Details

OriginalspracheEnglisch
Seiten (von - bis)349-364
Seitenumfang16
FachzeitschriftMolecular Plant Pathology
Jahrgang16
Ausgabenummer4
PublikationsstatusVeröffentlicht - 1 Mai 2015
Peer-Review-StatusJa

Externe IDs

PubMed 25135243

Schlagworte

Schlagwörter

  • Arabidopsis thaliana, Clubroot disease, Methyltransferase, Plasmodiophora brassicae, Salicylic acid

Bibliotheksschlagworte