A new embedded process for compartmentalized cell-free protein expression and on-line detection in microfluidic devices
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Just a drop. In the field of biological research and biotechnology, fast and efficient screening for particular properties of minute quantities down to single-particle level has become increasingly important. This work describes the implementation of a lab-on-chip device to generate small reaction containers, that is, a homodisperse water-in-oil emulsion, and cell-free expression of the green fluorescent protein inside the aqueous droplets. Detection of the autofluorescent protein was performed by confocal fluorescence spectroscopy.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 811-814 |
Seitenumfang | 4 |
Fachzeitschrift | ChemBioChem |
Jahrgang | 6 |
Ausgabenummer | 5 |
Publikationsstatus | Veröffentlicht - Mai 2005 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 15827950 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Fluorescence spectroscopy, High-throughput screening, Microfluidics, Microreactors, Molecular evolution