A Histopathologic and Immunohistochemical Study on Liquification of Human Adipose Tissue Ex Vivo

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Nina Fee Hübner - , Friedrich-Alexander-Universität Erlangen-Nürnberg (Autor:in)
  • Raymund E. Horch - , Friedrich-Alexander-Universität Erlangen-Nürnberg (Autor:in)
  • Elias Polykandriotis - , Sana Klinikum Hof (Autor:in)
  • Tilman T. Rau - , Friedrich-Alexander-Universität Erlangen-Nürnberg (Autor:in)
  • Adrian Dragu - , UniversitätsCentrum für Orthopädie, Unfall - und Plastische Chirurgie, Universitätsklinikum Leipzig , Klinikum St. Georg Leipzig (Autor:in)

Abstract

Background: This preliminary ex vivo study aimed to clarify the pathophysiologic mechanisms of fat tissue depletion by subcutaneous drug application. Therefore, the lipolytic effects of phosphatidylcholine plus deoxycholate (Lipostabil) (L) and of deoxycholate (DC) alone were compared with those of sodium chloride (NaCl) and hydrogen peroxide (H2O2) as control agents. The study enrolled 10 patients receiving abdominoplasty. The treatment periods for each sample and solution were 1, 3, 5, and 7 h. The samples were analyzed morphologically using hematoxylin-eosin (H&E) staining and also immunohistochemically using Caspase 3 and tumor necrosis factor (TNF)-alpha. Morphologic changes were seen best after 5 h of application time. Except for NaCl, all the samples in the H&E staining showed marked damage of adipocyte cell membranes, with the greatest disruption of normal cell architecture after hydrogen peroxide (H2O2) application. Immunohistochemistry using TNF-alpha showed positive results for the deoxycholate and Lipostabil samples and highly positive results for the H2O2 sample. Data from this study indicate that Lipostabil and deoxycholate induce pathways of cell necrosis involving TNF-alpha. These short-term experiments indicate that Lipostabil affects fat tissue in the way of a chemical-toxic destruction rather than via a physiologically induced, programmed cell death.

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Details

OriginalspracheEnglisch
Seiten (von - bis)976-984
Seitenumfang9
FachzeitschriftAesthetic plastic surgery
Jahrgang38
Ausgabenummer5
PublikationsstatusVeröffentlicht - 25 Sept. 2014
Peer-Review-StatusJa

Externe IDs

PubMed 25015550
ORCID /0000-0003-4633-2695/work/145698726

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • Abdominoplasty, Apoptosis, Deoxycholate, Lipolysis, Necrosis