A balancing act times two: sensing and regulating cell envelope homeostasis in Bacillus subtilis

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Georg Fritz - , Philipps-Universität Marburg (Autor:in)
  • Thorsten Mascher - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)

Abstract

Bacterial cell wall homeostasis is an intricately coordinated process that ensures that envelope integrity is maintained during cell growth and division, but can also adequately respond to growth-limiting conditions such as phosphate starvation. In Bacillus subtilis, biosynthesis of the two major cell wall components, peptidoglycan and anionic polymers, is controlled by a pair of paralogous two-component systems, WalRK and PhoPR respectively. Favorable growth conditions allow for a fast rate of cell wall biosynthesis (WalRK-ON) and the incorporation of the phosphate-containing anionic polymer teichoic acids (PhoPR-OFF). In contrast, growth-restricted cells under phosphate-limiting conditions reduce the incorporation of peptidoglycan building blocks (WalRK-OFF) and switch from the phosphate-containing teichoic acids to the phosphate-free anionic polymer teichuronic acid (PhoPR-ON). Botella et al. (2014) deepen our knowledge on the PhoPR system by identifying one signal that is perceived by its histidine kinase PhoR. In fast-growing cells, intracellular intermediates of teichoic acid biosynthesis are sensed by the cytoplasmic Per-Arnt-Sim domain as an indicator of favorable conditions, thereby inhibiting the autokinase activity of PhoR and keeping the system inactive. Depletion of teichoic acid building blocks under phosphate-limiting conditions relieves this inhibition, activates PhoPR-dependent signal transduction and hence the switch to teichuronic acid biosynthesis.

Details

OriginalspracheEnglisch
Seiten (von - bis)1201-1207
Seitenumfang7
FachzeitschriftMolecular microbiology
Jahrgang94
Ausgabenummer6
PublikationsstatusVeröffentlicht - Dez. 2014
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

Scopus 84914816105

Schlagworte

Schlagwörter

  • Bacillus subtilis/growth & development, Bacterial Proteins/genetics, Phosphates/metabolism, Teichoic Acids/metabolism

Bibliotheksschlagworte