Quantitative, nanometerscale information of dynamic processes in nanobiological transport systems can be obtained using standard microscopy equipment. Biomolecular motor-driven nanodevices are dynamic, soft systems exhibiting rapid energy flow and mechanical motion. Active movement is a key ability of biological nanomachines, and finds applications in a number of hybrid bio-nanodevices. Fluorescently-labeled MTs were deposited onto polystyrene layers of varying thickness on Si chips to calibrate optical system. Rhodamine-labeled MTs were polymerized from bovine brain tubulin in BRB80 buffer with 4 mM MgCl₂, I mM Mg-GTP, and 5% dimethylsulfoxide at 37°C. The fluorescence intensity of 30 MT segments of 2-μm length each on a glass surface, finding a standard deviation of less than 15%.