3 Screen islet cell autoantibody ELISA: A sensitive and specific ELISA for the combined measurement of autoantibodies to GAD65, to IA-2 and to ZnT8

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Marie Amoroso - , RSR Ltd (Autor:in)
  • Peter Achenbach - , Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt, Klinikum Rechts der Isar (MRI TUM), Deutsches Zentrum für Diabetesforschung (DZD e.V.) (Autor:in)
  • Michael Powell - , RSR Ltd (Autor:in)
  • Rebecca Coles - , RSR Ltd (Autor:in)
  • Monika Chlebowska - , RSR Ltd (Autor:in)
  • Lorraine Carr - , RSR Ltd (Autor:in)
  • Jadwiga Furmaniak - , RSR Ltd (Autor:in)
  • Marlon Scholz - , Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt, Klinikum Rechts der Isar (MRI TUM) (Autor:in)
  • Ezio Bonifacio - , Professur für Präklinische Stammzelltherapie und Diabetes, Deutsches Zentrum für Diabetesforschung (DZD e.V.) (Autor:in)
  • Anette-G Ziegler - , Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt, Klinikum Rechts der Isar (MRI TUM), Deutsches Zentrum für Diabetesforschung (DZD e.V.) (Autor:in)
  • Bernard Rees Smith - , RSR Ltd (Autor:in)

Abstract

3 Screen, a new ELISA for the combined measurement of autoantibodies to GAD65, to IA-2 and to ZnT8, has been developed and evaluated.

In the assay serum samples were incubated (overnight; 2–8 °C) in ELISA plate wells coated with GAD65, IA-2 and ZnT8 followed by a wash step and incubation with biotinylated GAD65, IA-2 and ZnT8 (1 h; 2–8 °C,). The assay was completed by addition of streptavidin-peroxidase and tetramethylbenzidine. Samples tested in the 3 Screen were also analysed in ELISAs and radiobinding assays for the three individual autoantibodies.

129/132 (98%) samples from newly diagnosed T1DM children and 1/100 non-diabetic children controls were positive in 3 Screen. There was good agreement between 3 Screen and the individual autoantibody assays. Dilution of positive samples showed good linearity characteristics. In the 2015 Islet Autoantibody Standardization Program 3 Screen achieved 94% sensitivity, 95.6% specificity and 0.948 area under curve by ROC analysis.

3 Screen provides a simple and sensitive method for combined measurement of three major diabetes associated autoantibodies in a single sample. The assay should be a useful tool for large scale population screening for individuals at risk of developing T1DM.

Details

OriginalspracheEnglisch
Seiten (von - bis)60-64
Seitenumfang5
FachzeitschriftClinica Chimica Acta
Jahrgang462
PublikationsstatusVeröffentlicht - 1 Nov. 2016
Peer-Review-StatusJa

Externe IDs

Scopus 84986592727
WOS 000387195400011
ORCID /0000-0002-8704-4713/work/164195886

Schlagworte

Forschungsprofillinien der TU Dresden

DFG-Fachsystematik nach Fachkollegium

Ziele für nachhaltige Entwicklung

Schlagwörter

  • Type 1 diabetes mellitus, Autoantibodies, GAD(65), IA-2, ZnT8, Population screening, Disease prediction

Bibliotheksschlagworte