Silver (I) N-Heterocyclic Carbenes Carbosilane Dendritic Systems and Their Imidazolium-Terminated Analogues as Antibacterial Agents: Study of Their Mode of Action

Research output: Contribution to journalResearch articleContributed

Contributors

  • Tamara Rodríguez-Prieto - , University of Alcalá (Author)
  • Philipp F Popp - , Institute of Microbiology (Author)
  • José Luis Copa-Patiño - , University of Alcalá (Author)
  • F Javier de la Mata - , University of Alcalá (Author)
  • Jesús Cano - , University of Alcalá (Author)
  • Thorsten Mascher - , Institute of Microbiology (Author)
  • Rafael Gómez - , University of Alcalá (Author)

Abstract

Spherical dendrimers and dendrons containing silver(I) N-heterocyclic carbenes (Ag(I)-NHC) and additionally bow-tie metal-free dendritic systems were synthesized in a simple and straightforward synthetic procedure and subsequently characterized. The antibacterial activity was evaluated, and in parallel, a comparative study with the cationic analogue precursors was performed to explore the effect of silver ions in the dendritic structure. Other parameters, such as topology, generation, and hydrophobicity, of the imidazole substituents were also studied. All these dendritic systems presented antibacterial activity against three different bacterial strains, two Gram-positive (Staphylococcus aureus and Bacillus subtilis) and one Gram-negative (Escherichia coli). Several assays were conducted to elucidate their mechanism of action against Bacillus subtilis, by using bacterial biosensors or specific probes and fluorescent proteins sensitive to changes in the cell membrane potential. These studies are specially focused on the role of the polyvalence of our systems containing silver atoms, which may provoke interesting effects in the mode of action.

Details

Original languageEnglish
Article number968
JournalPharmaceutics
Volume12
Issue number10
Publication statusPublished - 14 Oct 2020
Peer-reviewedNo

External IDs

PubMedCentral PMC7650833
Scopus 85092926819