Characterization of Total RNA, CD44, FASN, and PTEN mRNAs from Extracellular Vesicles as Biomarkers in Gastric Cancer Patients

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Philipp Rhode - , University Hospital Leipzig (Author)
  • Matthias Mehdorn - , University Hospital Leipzig (Author)
  • Orestis Lyros - , University Hospital Leipzig (Author)
  • Christoph Kahlert - , Department of Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus Dresden (Author)
  • Thomas Kurth - , Core Facility Electron Microscopy & Histology (Author)
  • Tom Venus - , Leipzig University (Author)
  • Katrin Schierle - , University Hospital Leipzig (Author)
  • Irina Estrela-Lopis - , Leipzig University (Author)
  • Boris Jansen-Winkeln - , University Hospital Leipzig (Author)
  • Florian Lordick - , University Hospital Leipzig (Author)
  • Ines Gockel - , University Hospital Leipzig (Author)
  • René Thieme - , University Hospital Leipzig (Author)

Abstract

In-depth characterization has introduced new molecular subtypes of gastric cancer (GC). To identify these, new approaches and techniques are required. Liquid biopsies are trendsetting and provide an easy and feasible method to identify and to monitor GC patients. In a prospective cohort of 87 GC patients, extracellular vesicles (EVs) were isolated from 250 µL of plasma. The total RNA was isolated with TRIZOL. The total RNA amount and the relative mRNA levels of CD44, PTEN, and FASN were measured by qRT-PCR. The isolation of EVs and their contained mRNA was possible in all 87 samples investigated. The relative mRNA levels of PTEN were higher in patients already treated by chemotherapy than in chemo-naïve patients. In patients who had undergone neoadjuvant chemotherapy followed by gastrectomy, a decrease in the total RNA amount was observed after neoadjuvant chemotherapy and gastrectomy, while FASN and CD44 mRNA levels decreased only after gastrectomy. The amount of RNA and the relative mRNA levels of FASN and CD44 in EVs were affected more significantly by chemotherapy and gastrectomy than by chemotherapy alone. Therefore, they are a potential biomarker for monitoring treatment response. Future analyses are needed to identify GC-specific key RNAs in EVs, which could be used for the diagnosis of gastric cancer patients in order to determine their molecular subtype and to accompany the therapeutic response.

Details

Original languageEnglish
JournalCancers
Volume13
Issue number23
Publication statusPublished - 27 Nov 2021
Peer-reviewedYes

External IDs

PubMedCentral PMC8656496
Scopus 85120858430
ORCID /0000-0001-5624-1717/work/142239001

Keywords

Sustainable Development Goals

Library keywords