Genetic influence on phenotypic differentiation in adult hippocampal neurogenesis

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Gerd Kempermann - , Professur für Regenerationsgenomik, Salk Institute for Biological Studies, Max-Delbrück-Centrum für Molekulare Medizin (MDC), Charité – Universitätsmedizin Berlin, Humboldt-Universität zu Berlin (Autor:in)
  • Fred H. Gage - , Salk Institute for Biological Studies (Autor:in)

Abstract

Regulation of adult hippocampal neurogenesis has different regulatory levels, including cell proliferation, survival and differentiation. Cell proliferation and survival are differentially influenced by inheritable traits and the genetic background determines which regulatory levels of adult hippocampal neurogenesis are preferentially involved in a neurogenic response to environmental stimuli. We here compared baseline adult neurogenesis in wild-derived strain Mus spretus and three inbred laboratory strains: A/J, C3H/HeJ and DBA/2J. Proliferation of was similar in the four strains, with the extremes being A/J, which had about 2100±570 (mean±S.D.) labeled newborn cells per dentate gyrus (after 6 days of bromodeoxyuridine injections), and DBA/2J, which had ∼1400±260. C3H/HeJ had ∼1500±600 and M. spretus had 1550±270. Survival of new cells after 4 weeks was 19% in A/J and DBA/2J, and 21% in M. spretus, but 37% in C3H/HeJ. Survival in C3H/HeJ was significantly different from DBA/2. Phenotypic analysis revealed that DBA/2J produced significantly fewer new neurons than A/J and C3H/HeJ (47% vs. 63% and 67%) but significantly more new astrocytes than A/J and C3H/HeJ (28% vs. 9% and 11%). In absolute terms there were 370±120 new neurons in C3H/HeJ, 250±60 in A/J, 130±50 in DBA/2J, and 190±130 in M. spretus. Our results indicate that regulation of adult hippocampal neurogenesis affects the level of phenotypic differentiation. At the present time it cannot be determined whether this regulation occurs by influencing cell fate decisions or by promoting selective survival.

Details

OriginalspracheEnglisch
Seiten (von - bis)1-12
Seitenumfang12
FachzeitschriftDevelopmental Brain Research
Jahrgang134
Ausgabenummer1-2
PublikationsstatusVeröffentlicht - 31 März 2002
Peer-Review-StatusJa

Externe IDs

PubMed 11947932
ORCID /0000-0002-5304-4061/work/161408164

Schlagworte

Schlagwörter

  • Bromodeoxyuridine, Progenitor cell, Rotarod, Stem cell, Water maze