Autofluorescence identifies highly phagocytic tissue-resident macrophages in mouse and human skin and cutaneous squamous cell carcinoma

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung


  • Pierre Bourdely - , Université Côte d'Azur (Autor:in)
  • Luciana Petti - , Université Côte d'Azur (Autor:in)
  • Sokchea Khou - , Université Côte d'Azur (Autor:in)
  • Aida Meghraoui-Kheddar - , Université Côte d'Azur (Autor:in)
  • Roxane Elaldi - , Université Côte d'Azur (Autor:in)
  • Julie Cazareth - , Université Côte d'Azur (Autor:in)
  • Noushine Mossadegh-Keller - , Aix-Marseille Université (Autor:in)
  • Julien Boyer - , Center for Regenerative Therapies Dresden (CRTD) (Autor:in)
  • Michael H Sieweke - , Center for Regenerative Therapies Dresden (CRTD), Professur für Stammzellforschung mit dem Schwerpunkt Zellbasierte Ansätze in der regenerativen Biomedizin, Aix-Marseille Université, Université de Bordeaux, INSERM - Institut national de la santé et de la recherche médicale, Centre d'Immunologie de Marseille-Luminy (CIML) (Autor:in)
  • Gilles Poissonnet - , Centre Hospitalier Universitaire (CHU) de Nice (Autor:in)
  • Anne Sudaka - , Centre Antoine Lacassagne (Autor:in)
  • Veronique M Braud - , Université Côte d'Azur (Autor:in)
  • Fabienne Anjuère - , Université Côte d'Azur (Autor:in)


Macrophages from human and mouse skin share phenotypic and functional features, but remain to be characterized in pathological skin conditions. Skin-resident macrophages are known to derive from embryonic precursors or from adult hematopoiesis. In this report, we investigated the origins, phenotypes and functions of macrophage subsets in mouse and human skin and in cutaneous squamous cell carcinoma (cSCC) using the spectral flow cytometry technology that enables cell autofluorescence to be considered as a full-fledged parameter. Autofluorescence identifies macrophage subsets expressing the CD206 mannose receptor in human peri-tumoral skin and cSCC. In mouse, all AF+ macrophages express the CD206 marker, a subset of which also displaying the TIM-4 marker. While TIM-4-CD206+ AF+ macrophages can differentiate from bone-marrow monocytes and infiltrate skin and tumor, TIM-4 identifies exclusively a skin-resident AF+ macrophage subset that can derive from prenatal hematopoiesis which is absent in tumor core. In mouse and human, AF+ macrophages from perilesional skin and cSCC are highly phagocytic cells contrary to their AF- counterpart, thus identifying autofluorescence as a bona fide marker for phagocytosis. Our data bring to light autofluorescence as a functional marker characterizing subsets of phagocytic macrophages in skin and cSCC. Autofluorescence can thus be considered as an attractive marker of function of macrophage subsets in pathological context.


FachzeitschriftFrontiers in immunology
PublikationsstatusVeröffentlicht - 17 Okt. 2022

Externe IDs

PubMedCentral PMC9619110
Scopus 85140976675



  • Adult, Humans, Animals, Mice, Carcinoma, Squamous Cell/pathology, Skin Neoplasms/pathology, Phagocytosis, Macrophages/pathology, Monocytes